Travellers – including holiday-makers – arriving back in Northern Ireland from Spain will have to face a mandatory 14-day quarantine.
And the measure will be imposed from midnight tonight (Saturday).
The Department of Health said the decision was made after a worrying surge in cases in Spain.
Health Minister Robin Swann said the decision was “not taken lightly”.
“I have always said I will move swiftly and take the necessary action to ensure our citizens are protected,” he said.
“I have spoken with my counterparts across the UK today and we agree that the recent upsurge in new positive cases in different parts of Spain are a cause for concern.
“As of midnight tonight, anyone returning or visiting Northern Ireland from Spain, including its islands will be required to quarantine for 14 days.
“I will continue to monitor the situation in Spain and other countries and make the changes to the regulations when necessary.”